Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of <i>Phaeocystis globosa</i> -- Journal of Oceanology and Limnology,2021,39(2):508-524

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	Qingchun ZHANG, Zhuang NIU, Jinxiu WANG, Chao LIU, Fanzhou KONG, Xiaokun HU, Jiayu ZHAO, Rencheng YU. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. Journal of Oceanology and Limnology, 2021, 39(2): 508-524

Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

Qingchun ZHANG^1,2,3, Zhuang NIU^1,2,3,4, Jinxiu WANG^1,2,3,4, Chao LIU^1,2,3,4, Fanzhou KONG^1,2,3, Xiaokun HU^1,2,3,4, Jiayu ZHAO^1,2,3,4, Rencheng YU^1,2,3,4

1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Ecology and Environmental Sciences, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;
3 Center of Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract:

Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world. The morphological, physiological, and biochemical characteristics are different among geographic strains, which can not be distinguished with nuclear ribosomal DNA markers at present. Therefore, the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1-D2 and ITS regions, and three chloroplast intergenic spacers (petN-trnS1, trnM1-psbA, and rbcS-rpl27) were analyzed and compared among 13 strains of P. globosa isolated from the Pacific Ocean and Atlantic Ocean in this study. In addition, the nucleotide polymorphisms of 28S rDNA D1-D2, ITS, and rbcS-rpl27 regions were evaluated in two P. globosa strains. The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1-D2 region and ITS region, but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer. A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer, which had the strongest distinction to geographic strains compared to those of 28S rDNA D1-D2 and ITS regions. In the phylogenetic tree of rbcS-rpl27 intergenic spacer, the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P. globosa, and then formed an independent clade, while the other Atlantic strains and all of Pacific strains joined up to build the other clade. It was implied that at least two genetically distant populations of P. globosa existed in the Atlantic coastal regions. This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P. globosa, and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P. globosa.

Key words: Phaeocystis globosa|chloroplast|DNA marker|phylogeny

Received: 2019-11-18 Revised: 2020-01-23

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Articles by Qingchun ZHANG

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Articles by Rencheng YU

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