Application of biofloc technology in recirculation <i>Artemia</i> culture system -- Journal of Oceanology and Limnology,2022,40(4):1669-1677

	Cite this paper:
	Xuejiao LIANG, Chi ZHANG, Dongdong DU, Meirong GAO, Liying SUI. Application of biofloc technology in recirculation Artemia culture system[J]. Journal of Oceanology and Limnology, 2022, 40(4): 1669-1677

Application of biofloc technology in recirculation Artemia culture system

Xuejiao LIANG¹, Chi ZHANG², Dongdong DU¹, Meirong GAO¹, Liying SUI¹

1 Key Laboratory of Marine Resource Chemistry and Food Technology(TUST), Ministry of Education;College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China;
2 Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China

Abstract:

Biofloc technology (BFT) improves water quality, and productivity of the farmed species through converting ammonium nitrogen to microbial protein, stabilizing microbial community, and reducing the production cost. In this study, a small-scale biofloc development unit was designed in combination of recirculation system (RAS) for Artemia culture. Artemia growth, water quality, and microbial composition of bioflocs in RAS were studied in comparison with in-situ batch culture (Glu). Glucose was added in RAS and Glu at C/N ratio of 10. The cultures without glucose addition, but with 50% daily water renewal (WRe) and without water renewal (NWRe) were considered as the controls. Artemia were cultured at 25 ℃ and salinity 30 for 24 days and fed formulated feed. The results showed that compared to the controls, Glu significantly improved the Artemia biomass, increased the biofloc volume, and reduced the content of total ammonia nitrogen (TAN), nitrite nitrogen (NO₂-N) and nitrate nitrogen (NO₃-N) in water column (P<0.05). In addition, RAS had similar results with Glu. High throughput sequencing analysis on biofloc microbial composition demonstrated that glucose supplement shaped the microbial community structure, and increased proportion of potential probiotic bacteria and suppressed pathogenic bacteria growth. Furthermore, we analyzed the relationship between the microbial composition of biofloc and environmental factors. Canonical correspondence analysis (CCA) indicated that inorganic nitrogen in culture water had great impact on biofloc microbial composition in NWRe and WRe, whilst the dissolved organic carbon (DOC) modified the microbial community in Glu and RAS. This study shows the advantages of BFT in Artemia culture and provides practical information for applying BFT-RAS in indoor Artemia culture.

Key words: biofloc technology|Artemia|water quality|microbial community structure

Received: 2021-05-06 Revised:

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Articles by Xuejiao LIANG

Articles by Chi ZHANG

Articles by Dongdong DU

Articles by Meirong GAO

Articles by Liying SUI

References:
Anand P S S, Kohli M P S, Kumar S, Sundaray J K, Roy S D, Venkateshwarlu G, Sinha A, Pailan G H.2014.Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon.
Aquaculture, 418-419:108-115, https://doi.org/10.1016/j.aquaculture.2013.09.051.
Anh N T N, Hien T T T, Mathieu W, van Hoa N, Sorgeloos P.2009.Effect of fishmeal replacement with Artemia biomass as a protein source in practical diets for the giant freshwater prawn Macrobrachium rosenbergii.
Aquaculture Research, 40(6):669-680, https://doi.org/10.1111/j.1365-2109.2008.02143.x.
Avnimelech Y.1999.Carbon/nitrogen ratio as a control element in aquaculture systems.Aquaculture, 176(3-4):227-235, https://doi.org/10.1016/S0044-8486(99)00085-X.
Caporaso J G, Lauber C L, Walters W A, Berg-Lyons D, Lozupone C A, Turnbaugh P J, Fierer N, Knight R.2011.Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample.Proceedings of the National Academy of Sciences of the United States of America, 108(S1):4516-4522, https://doi.org/10.1073/pnas.1000080107.
Cardona E, Gueguen Y, Magré K, Lorgeoux B, Piquemal D, Pierrat F, Noguier F, Saulnier D.2016.Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system.BMC Microbiology, 16(1):157, https://doi.org/10.1186/s12866-016-0770-z.
Chacur M M, Negreiros-Fransozo M L.2001.Spatial and seasonal distributions of Callinectes danae (Decapoda, Portunidae) in Ubatuba Bay, São Paulo, Brazil.Journal of Crustacean Biology, 21(2):414-425, https://doi.org/10.1163/20021975-99990142.
Chiu K H, Liu W S.2014.Dietary administration of the extract of Rhodobacter sphaeroides WL-APD911 enhances the growth performance and innate immune responses of seawater red tilapia (Oreochromis mossambicus×Oreochromis niloticus).Aquaculture, 418-419:32-38, https://doi.org/10.1016/j.aquaculture.2013.10.007.
Crab R, Chielens B, Wille M, Bossier P, Verstraete W.2010.The effect of different carbon sources on the nutritional value of bioflocs, a feed for Macrobrachium rosenbergii postlarvae.Aquaculture Research, 41(4):559-567, https://doi.org/10.1111/j.1365-2109.2009.02353.x.
Cytryn E, van Rijn J, Schramm A, Gieseke A, De Beer D, Minz D.2005.Identification of bacteria potentially responsible for oxic and anoxic sulfide oxidation in biofilters of a recirculating mariculture system.Applied and Environmental Microbiology, 71(10):6134-6141, https://doi.org/10.1128/aem.71.10.6134-6141.2005.
De Schryver P, Crab R, Defoirdt T, Boon N, Verstraete W.2008.The basics of bio-flocs technology:the added value for aquaculture.Aquaculture, 277(3-4):125-137, https://doi.org/10.1016/j.aquaculture.2008.02.019.
Edgar R C.2004.MUSCLE:multiple sequence alignment with high accuracy and high throughput.Nucleic Acids Research, 32(5):1792-1797, https://doi.org/10.1093/nar/gkh340.
Edgar R C.2013.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature Methods, 10(10):996-998, https://doi.org/10.1038/nmeth.2604.
Ekasari J, Rivandi D R, Firdausi A P, Surawidjaja E H, Zairin Jr M, Bossier P, De Schryver P.2015.Biofloc technology positively affects Nile tilapia (Oreochromis niloticus)larvae performance.Aquaculture, 441:72-77, https://doi.org/10.1016/j.aquaculture.2015.02.019.
Eryalcin K M.2018.Effects of different commercial feeds and enrichments on biochemical composition and fatty acid profile of rotifer (Brachionus plicatilis, Müller 1786) and Artemia franciscana.Turkish Journal of Fisheries and Aquatic Sciences, 18:81-90, https://doi.org/10.4194/1303-2712-v18_1_09.
Fischer H, Romano N, Renukdas N, Egnew N, Sinha A K, Ray A J.2020.The potential of rearing juveniles of bluegill, Lepomis macrochirus, in a biofloc system.Aquaculture Reports, 17:100398, https://doi.org/10.1016/j.aqrep.2020.100398.
Gao M R, Wang J, Ma G N, van Stappen G, Sui L Y.2017.Carbon supplementation and microbial management to stimulate Artemia biomass production in hypersaline culture conditions.Aquaculture Research, 48(3):1240-1250, https://doi.org/10.1111/are.12965.GB/T 12763.4-2007.2007.Specifications for oceanographic survey-Part 4:survey of chemical parameters in sea water.
Hari B, Madhusoodana K B, Varghese J T, Schrama J W, Verdegem M C J.2004.Effects of carbohydrate addition on production in extensive shrimp culture systems.Aquaculture, 241(1-4):179-194, https://doi.org/10.1016/j.aquaculture.2004.07.002.
Hjelm M, Riaza A, Formoso F, Melchiorsen J, Gram L.2004.Seasonal incidence of autochthonous antagonistic Roseobacter spp.and Vibrionaceae strains in a turbot larva (Scophthalmus maximus) rearing system.Applied and Environmental Microbiology, 70(12):7288-7294, https://doi.org/10.1128/AEM.70.12.7288-7294.2004.
Karunasagar I, Pai R, Malathi G R, Karunasagar I.1994.Mass mortality of Penaeus monodon larvae due to antibioticresistant Vibrio harveyi infection.Aquaculture, 128(1-4):203-209, https://doi.org/10.1016/0044-8486(94)90309-3.
Kuhn D D, Lawrence A L, Boardman G D, Patnaik S, Marsh L, Flick Jr G J.2010.Evaluation of two types of bioflocs derived from biological treatment of fish effluent as feed ingredients for Pacific white shrimp, Litopenaeus vannamei.Aquaculture, 303(1-4):28-33, https://doi.org/10.1016/j.aquaculture.2010.03.001.
Lavens P, Sorgeloos P.1996.Manual on the Production and Use of Live Food for Aquaculture.FAO, Rome.
Le T H, van Hoa N, Sorgeloos P, van Stappen G.2018.Artemia feeds:a review of brine shrimp production in the Mekong Delta, Vietnam.Reviews in Aquaculture, 11(4):1169-1175, https://doi.org/10.1111/raq.12285.
Lozupone C A, Hamady M, Kelley S T, Knight R.2007.Quantitative and qualitative β diversity measures lead to different insights into factors that structure microbial communities.Applied and Environmental Microbiology, 73(5):1576-1585, https://doi.org/10.1128/AEM.01996-06.
Lozupone C, Lladser M E, Knights D, Stombaugh J, Knight R.2011.UniFrac:an effective distance metric for microbial community comparison.The ISME Journal, 5(2):169-172, https://doi.org/10.1038/ismej.2010.133.
Makridis P, Vadstein O.1999.Food size selectivity of Artemia franciscana at three developmental stages.Journal of Plankton Research, 21(11):2191-2201, https://doi.org/10.1093/plankt/21.11.2191.
Miura Y, Hiraiwa M N, Ito T, Watanabe Y, Okabe S.2007.Bacterial community structures in MBRs treating municipal wastewater:relationship between community stability and reactor performance.Water Research, 41(3):627-637, https://doi.org/10.1016/j.watres.2006.11.005.
Moriarty D J W.1998.Control of luminous Vibrio species in penaeid aquaculture ponds.Aquaculture, 164(1-4):351-358, https://doi.org/10.1016/s0044-8486(98)00199-9.
Nevejan N, De Schryver P, Wille M, Dierckens K, Baruah K, van Stappen G.2018.Bacteria as food in aquaculture:do they make a difference? Reviews in Aquaculture, 10(1):180-212, https://doi.org/10.1111/raq.12155.
Rosenberger S, Krüger U, Witzig R, Manz W, Szewzyk U, Kraume M.2002.Microbiological aspects of a bioreactor with submerged membranes for aerobic treatment of municipal wastewater.Water Research, 36(2):413-420, https://doi.org/10.1016/S0043-1354(01)00223-8.
Sakami T, Fujioka Y, Shimoda T.2008.Comparison of microbial community structures in intensive and extensive shrimp culture ponds and a mangrove area in Thailand.Fisheries Science, 74(4):889-898, https://doi.org/10.1111/j.1444-2906.2008.01604.x.
Sandoval-Vargas L Y, Jiménez-Amaya M N, Rodríguez-Pulido J, Guaje-Ramírez D N, Ramírez-Merlano J A, MedinaRobles V M.2020.Applying biofloc technology in the culture of juvenile of Piaractus brachypomus (Cuvier, 1818):effects on zootechnical performance and water quality.Aquaculture Research, 51(9):3865-3878, https://doi.org/10.1111/are.14734.
Schneider O, Sereti V, Eding E H, Verreth J A J.2005.Analysis of nutrient flows in integrated intensive aquaculture systems.Aquacultural Engineering, 32(3-4):379-401, https://doi.org/10.1016/j.aquaeng.2004.09.001.
Sorgeloos P, Dhert P, Candreva P.2001.Use of the brine shrimp, Artemia spp., in marine fish larviculture.Aquaculture, 200(1-2):147-159, https://doi.org/10.1016/s0044-8486(01)00698-6.
Sui L Y, Wang J, Nguyen V H, Sorgeloos P, Bossier P, van Stappen G.2013.Increased carbon and nitrogen supplementation in Artemia culture ponds results in higher cyst yields.Aquaculture International, 21(6):1343-1354, https://doi.org/10.1007/s10499-013-9637-6.
Toi H T, Boeckx P, Sorgeloos P, Bossier P, van Stappen G.2013.Bacteria contribute to Artemia nutrition in algaelimited conditions:a laboratory study.Aquaculture, 388-391:1-7, https://doi.org/10.1016/j.aquaculture.2013.01.005.
Wagner M, Amann R, Lemmer H, Schleifer K H.1993.Probing activated sludge with oligonucleotides specific for proteobacteria:inadequacy of culture-dependent methods for describing microbial community structure.Applied and Environmental Microbiology, 59(5):1520-1525, https://doi.org/10.1128/AEM.59.5.1520-1525.1993.
Wang S Y, Cui X P, Xu R Y, Gao M R, Sui L Y.2019.Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition.Journal of Oceanology and Limnology, 37(5):1768-1776, https://doi.org/10.1007/s00343-019-8288-5.
Wasielesky Jr W, Atwood H, Stokes A, Browdy C L.2006.Effect of natural production in a zero exchange suspended microbial floc based super-intensive culture system for white shrimp Litopenaeus vannamei.Aquaculture, 258(1-4):396-403, https://doi.org/10.1016/j.aquaculture.2006.04.030.
Wei Y F, Liao S A, Wang A L.2016.The effect of different carbon sources on the nutritional composition, microbial community and structure of bioflocs.Aquaculture, 465:88-93, https://doi.org/10.1016/j.aquaculture.2016.08.040.
Xu W J, Xu Y, Su H C, Hu X J, Xu Y N, Li Z J, Wen G L, Cao Y C.2021.Production performance, inorganic nitrogen control and bacterial community characteristics in a controlled biofloc-based system for indoor and outdoor super-intensive culture of Litopenaeus vannamei.Aquaculture, 531:735749, https://doi.org/10.1016/j.aquaculture.2020.735749.