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Liying SUI, Xiaocui LIU, Namin PAN, Xue LIU, Meirong GAO. Halomonas-PHB protects gnotobiotic Artemia against Vibrio and modifies Artemia gut microbiota in xenic culture conditions[J]. Journal of Oceanology and Limnology, 2023, 41(4): 1292-1299

Halomonas-PHB protects gnotobiotic Artemia against Vibrio and modifies Artemia gut microbiota in xenic culture conditions

Liying SUI1,2, Xiaocui LIU1,2, Namin PAN1,2, Xue LIU1,2, Meirong GAO1,2
1 Key Laboratory of Marine Resource Chemistry and Food Technology(TUST), Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China;
2 Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
The prokaryotic cell storage compound ploy-β-hydroxybutyrate (PHB) has been considered as prebiotics that can be applied in aquaculture. In this paper, the dietary effect of a PHB-accumulating Halomonas strain (HM·PHB) identified from our previous work were studied in Artemia under gnotobiotic and xenic culture conditions, in comparison of Halomonas without PHB accumulation (HM) and microalgae Isochrysis (ISO) feeding. Under gnotobiotic condition, both HM·PHB and HM served as sole food supporting Artemia survival. Although both HM·PHB and HM feeding had no significant difference on Artemia survival percentage (P>0.05), HM·PHB significantly improved their resistance against Vibrio anguillarum challenge (P<0.05). Mass Artemia culture were further performed in xenic condition. Compared to ISO, HM·PHB feeding protected Artemia against V. anguillarum challenge (P<0.05), and HM·PHB and HM feeding resulted in increased T-AOC, pepsin, T-SOD and CAT activities (P<0.05). High throughput sequencing analysis showed that HM·PHB and HM feeding resulted in a lower Artemia gut microbial diversity (P<0.05), and modified the gut microbial community by remarkably reducing the Vibrio proportion. The outcome of the paper confirmed the beneficial effect of Halomonas-PHB in Artemia culture, which supports the use of Halomonas-PHB in the production of bio-secured live feed Artemia.
Key words:    Halomonas-ploy-β-hydroxybutyrate (Halomonas-PHB)|Artemia|survival|pathogen resistance|gut microbiota   
Received: 2022-04-07   Revised:
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