Cite this paper:
Quanchao WANG, Ying LIU, Zirui PENG, Linlin CHEN, Baoquan LI. Genetic diversity and population structure of the sea star Asterias amurensis in the northern coast of China[J]. Journal of Oceanology and Limnology, 2023, 41(4): 1593-1601

Genetic diversity and population structure of the sea star Asterias amurensis in the northern coast of China

Quanchao WANG1,3, Ying LIU1,2, Zirui PENG1,4, Linlin CHEN1,3, Baoquan LI1,3
1 Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
4 Ocean School, Yantai University, Yantai 264005, China
The sea star Asterias amurensis is widely viewed as a severe “marine pest” because of its broad feeding habits. Over the past few decades, A. amurensis undergoes massive and sporadic population outbreaks worldwide, causing extensive economic and ecological losses to the local aquaculture industry and marine ecosystem. Understanding the genetic diversity and population structure of A. amurensis can provide vital information for resource management. By analyzing the polymorphism of the mitochondrial cytochrome C oxidase subunit I (COI) gene and ten simple sequence repeat (SSR) microsatellites markers, the genetic diversity and population structure of A. amurensis of four populations along the northern coast of China was uncovered. A total of 36 haplotypes were identified, and a main haplotype was found in four populations. The Qingdao (QD) population displayed the highest genetic diversity among all the populations. The AMOVA and pairwise Fst showed that there was small but statistically significant population differentiation among the four populations, especially between QD and Weihai (WH). Moreover, the principal component analysis (PCA) and admixture analysis showed that several individuals in Yantai (YT) and Dalian (DL) had little genetic association with other individuals. Overall, this study provided useful information of the genetic diversity and population structure of A. amurensis and will contribute to the resource management of A. amurensis in China.
Key words:    Asterias amurensis|cytochrome C oxidase subunit I (COI)|simple sequence repeat (SSR)|population structure|China seas   
Received: 2021-12-28   Revised:
PDF (1114 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by Quanchao WANG
Articles by Ying LIU
Articles by Zirui PENG
Articles by Linlin CHEN
Articles by Baoquan LI
Addison J A, Hart M W. 2005. Spawning, copulation and inbreeding coefficients in marine invertebrates. Biology Letters, 1(4): 450-453, 0353.
Ashe J L, Wilson A B. 2020. Navigating the southern seas with small fins: genetic connectivity of seahorses(Hippocampus abdominalis) across the Tasman Sea.Journal of Biogeography, 47(1): 207-219,
Balloux F, Lugon-Moulin N. 2002. The estimation of population differentiation with microsatellite markers.Molecular Ecology, 11(2): 155-165,
Bruvo R, Michiels N K, D’Souza T G et al. 2004. A simple method for the calculation of microsatellite genotype distances irrespective of ploidy level. Molecular Ecology, 13(7): 2101-2106, 2004.02209.x.
Byrne M, Morrice M G, Wolf B. 1997. Introduction of the northern Pacific asteroid Asterias amurensis to Tasmania:reproduction and current distribution. Marine Biology, 127(4): 673-685,
Chen B, Yu K F, Yao Q C et al. 2021. Insights into the environmental impact on genetic structure and larval dispersal of crown-of-thorns starfish in the South China Sea. Frontiers in Marine Science, 8: 728349,
Cho E S, Jung C G, Sohn S G et al. 2007. Population genetic structure of the ark shell Scapharca broughtonii Schrenck from Korea, China, and Russia based on COI gene sequences. Marine Biotechnology, 9(2): 203-216,
Clark L V, Jasieniuk M. 2011. POLYSAT: an R package for polyploid microsatellite analysis. Molecular Ecology Resources, 11(3): 562-566,
Do C, Waples R S, Peel D et al. 2014. NeEstimator v2:re-implementation of software for the estimation of contemporary effective population size (Ne) from genetic data. Molecular Ecology Resources, 14(1): 209-214,
Drinan D P, Gruenthal K M, Canino M F et al. 2018.Population assignment and local adaptation along an isolation-by-distance gradient in Pacific cod (Gadus macrocephalus). Evolutionary Applications, 11(8): 1448-1464,
Excoffier L, Lischer H E L. 2010.Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3): 564-567,
Fontaine M C, Baird S J, Piry S et al. 2007. Rise of oceanographic barriers in continuous populations of a cetacean: the genetic structure of harbour porpoises in Old World waters. BMC Biology, 5(1): 30,
Garcia-Cisneros A, Palacín C, Ben Khadra Y et al. 2016.Low genetic diversity and recent demographic expansion in the red starfish Echinaster sepositus (Retzius 1816).Scientific Reports, 6(1): 33269,
Goudet J. 2005. HIERFSTAT, a package for R to compute and test hierarchical F-statistics. Molecular Ecology Notes, 5(1): 184-186, 2004.00828.x.
Grant W A S, Bowen B W. 1998. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation. Journal of Heredity, 89(5): 415-426,
Hall T A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41(2): 95-98.
Horoiwa M, Nakamura T, Yuasa H et al. 2022. Integrated population genomic analysis and numerical simulation to estimate larval dispersal of Acanthaster cf. solaris between Ogasawara and other Japanese regions. Frontiers in Marine Science, 8: 688139,
Hutson K S, Ross D J, Day R W et al. 2005. Australian scallops do not recognise the introduced predatory seastar Asterias amurensis. Marine Ecology Progress Series, 298: 305-309,
Iguchi A, Tada I, Nagano A J et al. 2021. Genetic structure of Pacific crown-of-thorns starfish (Acanthaster cf. solaris) in southern Japan based on genome-wide RADseq analysis. Coral Reefs, 40(4): 1379-1385,
Kashenko S D. 2005. Responses of embryos and larvae of the starfish Asterias amurensis to changes in temperature and salinity. Russian Journal of Marine Biology, 31(5):294-302,
Larson W A, Seeb L W, Everett M V et al. 2014. Genotyping by sequencing resolves shallow population structure to inform conservation of Chinook salmon (Oncorhynchus tshawytscha). Evolutionary Applications, 7(3): 355-369,
Lee C H, Ryu T K, Choi J W. 2004. Effects of water temperature on embryonic development in the northern Pacific asteroid, Asterias amurensis, from the southern coast of Korea. Invertebrate Reproduction & Development, 45(2): 109-116, 9652580.
Leigh J W, Bryant D. 2015. POPART: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6(9): 1110-1116,
Li B Q, Zhou Z Q, Li B J et al. 2018. Size distribution of individuals in the population of Asterias amurensis(Echinodermata: Asteroidea) and its reproductive cycle in China. Acta Oceanologica Sinica, 37(6): 96-103,
Li H Y, Li X, Sun Y Q et al. 2014. Analysis and evaluation of nutritive composition of Asterias amurensis. Food Science, 35(21): 207-211, (in Chinese with English abstract)
Ligges U, Mächler M. 2003. Scatterplot3d-an R package for visualizing multivariate data. Journal of Statistical Software, 8(11): 1-20,
Liu G, Zhou L Z. 2017. Population genetic structure and molecular diversity of the red swamp crayfish in China based on mtDNA COI gene sequences. Mitochondrial DNA Part A, 28(6): 860-866,
Meriam T, Wafa T, Khawla T et al. 2015. Genetic diversity and population structure of Sepia officinalis from the Tunisian cost revealed by mitochondrial COI sequences. Molecular Biology Reports, 42(1): 77-86,
Nie H T, Niu H B, Zhao L Q et al. 2015. Genetic diversity and structure of Manila clam (Ruditapes philippinarum) populations from Liaodong peninsula revealed by SSR markers. Biochemical Systematics and Ecology, 59: 116-125,
Paradis E. 2010.Pegas: an R package for population genetics with an integrated-modular approach. Bioinformatics, 26(3): 419-420,
Richardson M F, Stanley A M, Sherman C D H. 2012.Development of novel microsatellite markers for the invasive Northern Pacific seastar, Asterias amurensis.Conservation Genetics Resources, 4(2): 327-330,
Rogers A R, Harpending H. 1992. Population growth makes waves in the distribution of pairwise genetic differences.Molecular Biology and Evolution, 9(3): 552-569,
Ross D J, Johnson C R, Hewitt C L. 2002. Impact of introduced seastars Asterias amurensis on survivorship of juvenile commercial bivalves Fulvia tenuicostata.Marine Ecology Progress Series, 241: 99-112,
Ross D J, Johnson C R, Hewitt C L et al. 2004. Interaction and impacts of two introduced species on a softsediment marine assemblage in SE Tasmania. Marine Biology, 144(4): 747-756,
Rozas J, Ferrer-Mata A, Sánchez-DelBarrio J C et al. 2017.DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution, 34(12): 3299-3302,
Scherer N. 2018. Northern Pacific Seastar, Asterias amurensis. In: Ackerson C ed. Aquatic Invasions: Causes, Consequences, and Solutions. University of New England. p.33-38.
Wang M S, Wang W J, Xiao G X et al. 2016. Genetic diversity analysis of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay based on an SSR marker. Acta Oceanologica Sinica, 35(8): 51-56,
Ward R D, Andrew J. 1995. Population genetics of the northern Pacific seastar Asterias amurensis (Echinodermata:Asteriidae): allozyme differentiation among Japanese, Russian, and recently introduced Tasmanian populations.Marine Biology, 124(1): 99-109,
Wright S. 1978. Evolution and the Genetics of Populations, Volume 4: Variability within and Among Natural Populations. University of Chicago Press, Chicago.
Zhang Q, Zhang C S, Yu Y et al. 2020. Characteristic analysis of simple sequence repeats in the ridgetail white prawn Exopalaemon carinicauda genome and its application in parentage assignment. Journal of the World Aquaculture Society, 51(3): 690-701,
Zheng J H, Nie H T, Yang F et al. 2019. Genetic variation and population structure of different geographical populations of Meretrix petechialis based on mitochondrial gene COI. Journal of Genetics, 98(3): 68,
Copyright © Haiyang Xuebao