Cite this paper:
Qingshang SONG, Yue XUE, Yanying ZHANG, Jiehui YIN, Pingping SHEN. Diversity and geographical distribution of haptophyte Phaeocystis in the Chinese seas based on metabarcoding analysis[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2197-2207

Diversity and geographical distribution of haptophyte Phaeocystis in the Chinese seas based on metabarcoding analysis

Qingshang SONG, Yue XUE, Yanying ZHANG, Jiehui YIN, Pingping SHEN
Ocean School, Yantai University, Yantai 264005, China
Phaeocystis is an important bloom-forming species and over 100 blooms have occurred since 1997 along the Chinese coasts, while only the species P. globosa was described. In this project, a total of 246 phytoplankton samples collected from the Bohai Sea, the Yellow Sea, and the East China Sea in April 2021 were analyzed to evaluate the species diversity and geographical distribution of the genus Phaeocystis based on metabarcoding 18S rDNA sequence. Four described Phaeocystis species were recognized, including P. globosa, P. pouchetii, P. jahnii, and P. cordata, and each has distinguished geographical distribution characteristics. P. globosa was the most widespread and abundant species, and P. pouchetii was found in the Bohai Sea and the northern Yellow Sea with substantially elevated abundance. P. cordata was also a widespread species, but its abundance was relatively low, while P. jahnii gathered only in the southeastern East China Sea. Water temperature, phosphate as well as ammonium contents were found to be associated with the abundance of P. globosa, P. pouchetii, and P. jahnii significantly. Moreover, two uncharacterized Phaeocystis species were detected in the Chinese seas, indicating the diversity of the genus remains underestimated worldwide.
Key words:    Haptophyta|Phaeocystis|species diversity|distribution|Chinese seas|amplicon sequence variant   
Received: 2022-06-19   Revised:
PDF (4956 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by Qingshang SONG
Articles by Yue XUE
Articles by Yanying ZHANG
Articles by Jiehui YIN
Articles by Pingping SHEN
Andersen R A, Bailey J C, Decelle J et al.2015.Phaeocystis rex sp.nov.(Phaeocystales, Prymnesiophyceae):a new solitary species that produces a multilayered scale cell covering.European Journal of Phycology, 50(2):207-222,
Borkman D G, Libby P S, Mickelson M J et al.2016.Variability of winter-spring bloom Phaeocystis pouchetii abundance in Massachusetts Bay.Estuaries and Coasts, 39(4):1084-1099,
Callahan B J, McMurdie P J, Rosen M J et al.2016.DADA2:high-resolution sample inference from Illumina amplicon data.Nature Methods, 13(7):581-583,
Chen S F, Zhou Y Q, Chen Y R et al.2018.Fastp:an ultra-fast all-in-one FASTQ preprocessor.Bioinformatics, 34(17):i884-i890,
Chen Y Q, Wang N, Zhang P et al.2002.Molecular evidence identifies bloom-forming Phaeocystis (Prymnesiophyta) from coastal waters of southeast China as Phaeocystis globosa.Biochemical Systematics and Ecology, 30(1):15-22,
de Vargas C, Audic S, Henry N et al.2015.Eukaryotic plankton diversity in the sunlit ocean.Science, 348(6237):1261605,
Decelle J, Probert I, Bittner L et al.2012.An original mode of symbiosis in open ocean plankton.Proceedings of the National Academy of Sciences of the United States of America, 109(44):18000-18005,
Doan-Nhu H, Larsen J.2010.Haptophyte algae of Vietnamese waters.The orders Phaeocystales, Prymnesiales and Isochrysidales (Prymnesiophyceae).Nova Hedwigia, 91(1-2):193-222,
Gran-Stadniczeñko S, Šupraha L, Egge E D et al.2017.Haptophyte diversity and vertical distribution explored by 18S and 28S ribosomal RNA gene metabarcoding and scanning electron microscopy.Journal of Eukaryotic Microbiology, 64(4):514-532,
Hai D N, Lam N N, Dippner J W.2010.Development of Phaeocystis globosa blooms in the upwelling waters of the South Central coast of Viet Nam.Journal of Marine Systems, 83(3-4):253-261,
Hansen E, Eilertsen H C, Ernstsen A et al.2003.Anti-mitotic activity towards sea urchin embryos in extracts from the marine haptophycean Phaeocystis pouchetii (Hariot) Lagerheim collected along the coast of northern Norway.Toxicon, 41(7):803-812,
Kumar S, Stecher G, Li M et al.2018.MEGA X:molecular evolutionary genetics analysis across computing platforms.Molecular Biology and Evolution, 35(6):1547-1549,
Lancelot C, Gypens N, Billen G et al.2007.Testing an integrated river-ocean mathematical tool for linking marine eutrophication to land use:the Phaeocystis-dominated Belgian coastal zone (Southern North Sea) over the past 50 years.Journal of Marine Systems, 64(1-4):216-228,
Lange M, Chen Y Q, Medlin L K.2002.Molecular genetic delineation of Phaeocystis species (Prymnesiophyceae) using coding and non-coding regions of nuclear and plastid genomes.European Journal of Phycology, 37(1):77-92,
Li D M, Xue Y, Song Q S et al.2022.First report on large-scale Phaeocystis globosa bloom in the southern Yellow Sea, China.Frontiers in Marine Science, 9:880984,
Lin Y C, Chung C C, Gong G C et al.2014.Diversity and abundance of haptophytes in the East China Sea.Aquatic Microbial Ecology, 72(3):227-240,
Magoč T, Salzberg S L.2011.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics, 27(21):2957-2963,
Medlin L, Zingone A.2007.A taxonomic review of the genus Phaeocystis.Biogeochemistry, 83(1-3):3-18,
Moestrup Ø.1979.Identification by electron microscopy of marine nanoplankton from New Zealand, including the description of four new species.New Zealand Journal of Botany, 17(1):61-95,
Novarino G.2014.Nanoplankton protists from the western Mediterranean Sea.III.Morphological diversity of Phaeocystis unicells (Prymnesiophyceae=Prymnesiida p.p).The Quekett Journal of Microscopy, 42(3):175-192.
Parsons T R, Maita Y, Lalli C M.1984.A Manual of Chemical and Biological Methods for Seawater Analysis.Pergamon Press, New York.
Peng X C, Yang W D, Liu J S et al.2005.Characterization of the hemolytic properties of an extract from Phaeocystis globosa Scherffel.Journal of Integrative Plant Biology, 47(2):165-171,
Qu L Y, Lv S H, Gao C L et al.2008.Structure and sequence analysis of 18s rDNA and ITS gene of Phaeocystis isolate from the Bohai Sea.Advances in Marine Science, 26(2):200-206, Chinese with English abstract)
Rousseau V, Chrétiennot-Dinet M J, Jacobsen A et al.2007.The life cycle of Phaeocystis:state of knowledge and presumptive role in ecology.Biogeochemistry, 83(1-3):29-47,
Rousseau V, Lantoine F, Rodriguez F et al.2013.Characterization of Phaeocystis globosa (Prymnesiophyceae), the blooming species in the Southern North Sea.Journal of Sea Research, 76:105-113,
Saiz E, Calbet A, Isari S et al.2013.Zooplankton distribution and feeding in the Arctic Ocean during a Phaeocystis pouchetii bloom.Deep Sea Research Part I:Oceanographic Research Papers, 72:17-33,
Schoemann V, Becquevort S, Stefels J et al.2005.Phaeocystis blooms in the global ocean and their controlling mechanisms:a review.Journal of Sea Research, 53(1-2):43-66,
Shen P P, Qi Y Z.2021.Research progress on species diversity and distribution of the genus Phaeocystis.Oceanologia et Limnologia Sinica, 52(1):1-15, Chinese with English abstract)
Shen P P, Qi Y Z, Ou L J.2018.Phaeocystis globosa in coastal China:taxonomy, distribution, and its blooms.Marine Sciences, 42(10):146-162, Chinese with English abstract)
Shih C Y, Lu H M, Gong G C et al.2019.High diversity of haptophytes in the East China Sea revealed by next-generation sequencing and scanning electron microscopy.Journal of Oceanography, 75(4):305-317,
Simo-Matchim A G, Gosselin M, Poulin M et al.2017.Summer and fall distribution of phytoplankton in relation to environmental variables in Labrador fjords, with special emphasis on Phaeocystis pouchetii.Marine Ecology Progress Series, 572:19-42,
Smith W O Jr, Liu X, Tang K W et al.2014.Giantism and its role in the harmful algal bloom species Phaeocystis globosa.Deep Sea Research Part II:Topical Studies in Oceanography, 101:95-106,
Smith W O Jr, Zhang W G, Hirzel A et al.2021.A regional, early spring bloom of Phaeocystis pouchetii on the New England continental shelf.Journal of Geophysical Research, 126(2):e2020JC016856,
Song H, Chen Y, Liu F et al.2021.Large differences in the haptophyte Phaeocystis globosa mitochondrial genomes driven by repeat amplifications.Frontiers in Microbiology, 12:676447,
Song L, Wu J, Liu W D et al.2016.Diversity of marine nanophytoplankton and picophytoplankton in Changxing Island offshore waters of Bohai Sea.Research of Environmental Sciences, 29(11):1635-1642, Chinese with English abstract)
Stefels J, Steinke M, Turner S et al.2007.Environmental constraints on the production and removal of the climatically active gas dimethylsulphide (DMS) and implications for ecosystem modelling.Biogeochemistry, 83(1-3):245-275,
Stoeck T, Bass D, Nebel M et al.2010.Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water.Molecular Ecology, 19:21-31,
Tu J B, Zhang Q F, Xu Y S et al.2011.Preliminary analysis of Phaeocystis globosa Scherffel red tide in Tianjin coastal sea areas of Bohai Bay.Marine Science Bulletin, 30(3):334-337, Chinese with English abstract)
Verity P G, Brussaard C P, Nejstgaard J C et al.2007.Current understanding of Phaeocystis ecology and biogeochemistry, and perspectives for future research.Biogeochemistry, 83(1-3):311-330,
Wang K, Chen B H, Gao Y H et al.2021.Harmful algal blooms caused by Phaeocystis globosa from 1997 to 2018 in Chinese coastal waters.Marine Pollution Bulletin, 173:112949,
Wang X D, Wang Y, Smith W O Jr.2011.The role of nitrogen on the growth and colony development of Phaeocystis globosa (Prymnesiophyceae).European Journal of Phycology, 46(3):305-314,
Wassmann P, Ratkova T, Reigstad M.2005.The contribution of single and colonial cells of Phaeocystis pouchetii to spring and summer blooms in the north-eastern North Atlantic.Harmful Algae, 4(5):823-840,
Wu W X, Wang L, Liao Y et al.2015.Microbial eukaryotic diversity and distribution in a river plume and cyclonic eddy-influenced ecosystem in the South China Sea.MicrobiologyOpen, 4(5):826-840,
Xu Y X, Zhang T, Zhou J.2019.Historical occurrence of algal blooms in the northern Beibu Gulf of China and implications for future trends.Frontiers in Microbiology, 10:451,
Zhang Q C, Niu Z, Wang J X et al.2021.Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa.Journal of Oceanology and Limnology, 39(2):508-524,
Zingone A, Chrétiennot-Dinet M J, Lange M et al.1999.Morphological and genetic characterization of Phaeocystis cordata and P.jahnii (Prymnesiophyceae), two new species from the Mediterranean Sea.Journal of Phycology, 35(6):1322-1337,
Zingone A, Forlani G, Percopo I et al.2011.Morphological characterization of Phaeocystis antarctica (Prymnesiophyceae).Phycologia, 50(6):650-660,
Copyright © Haiyang Xuebao