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Li YIN, Ying XU, Desheng KONG, Juan WANG, Kaipian SHI, Yong ZHANG, Huan HE, Shaogui YANG, Lixiao NI, Shiyin LI. Role of extracellular polymeric substances in resistance to allelochemical stress on Microcystis aeruginsosa and its mechanism[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2219-2231

Role of extracellular polymeric substances in resistance to allelochemical stress on Microcystis aeruginsosa and its mechanism

Li YIN1, Ying XU1, Desheng KONG1, Juan WANG1, Kaipian SHI1, Yong ZHANG2, Huan HE1, Shaogui YANG1, Lixiao NI3, Shiyin LI1,4
1 School of Environment, Nanjing Normal University, Nanjing 210023, China;
2 Department of Geological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA;
3 Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes of Ministry of Education;School of Environment, Hohai University, Nanjing 210098, China;
4 Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China
Using allelochemicals to suppress cyanobacteria growth is a prospective method for its high efficiency and ecological safety. However, the suppression efficiency is affected inevitably by the extracellular polymeric substances (EPS) produced by cyanobacteria, and the knowledge about the roles of EPS in resistance to allelochemical stress is scarce. For the study, two typical anti-cyanobacterial allelochemicals were adopted to investigate the role of EPS in resistance to allelochemical stress on Microcystis aeruginosa. Results show that EPS was crucial in alleviating the toxicity of allelochemicals to algae, especially in stabilizing the metabolism and photosynthetic activity of algal cells. The aggregation rate of algal cells increased with the increase of EPS secretion, which alleviated the stress of allelopathy. Tryptophan proteins and humic acids in EPS provided a binding site for allelochemicals, and the EPS-allelochemicals complex were formed by chemical bonding. This study improved our comprehension of the role of EPS in algal inhibition by allelochemicals.
Key words:    allelochemicals    extracellular polymeric substances    cyanobacteria    Microcystis aeruginosa    allelopathy   
Received: 2022-09-13   Revised:
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