Temporal characteristics of algae-denitrifying bacteria co-occurrence patterns and denitrifier assembly in epiphytic biofilms on submerged macrophytes in Caohai Lake, SW China -- Journal of Oceanology and Limnology,2023,41(6):2276-2291

	Cite this paper:
	Pinhua XIA, Guoqing LI, Xianfei HUANG, Lei SHI, Xin DU, Tao LIN. Temporal characteristics of algae-denitrifying bacteria co-occurrence patterns and denitrifier assembly in epiphytic biofilms on submerged macrophytes in Caohai Lake, SW China[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2276-2291

Temporal characteristics of algae-denitrifying bacteria co-occurrence patterns and denitrifier assembly in epiphytic biofilms on submerged macrophytes in Caohai Lake, SW China

Pinhua XIA, Guoqing LI, Xianfei HUANG, Lei SHI, Xin DU, Tao LIN

Guizhou Province Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China

Abstract:

Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats. However, little is known about the connection between algae and denitrifying bacteria and their assembly processes in epiphytic biofilms. Epiphytic biofilms were collected from submerged macrophytes (Patamogeton lucens and Najas marina L.) in the Caohai Lake, Guizhou, SW China, from July to November 2020 to: (1) investigate the impact of abiotic and biotic variables on denitrifying bacterial communities; (2) investigate the temporal variation of the algae-denitrifying bacteria co-occurrence networks; and (3) determine the contribution of deterministic and stochastic processes to the formation of denitrifying bacterial communities. Abiotic and biotic factors influenced the variation in the denitrifying bacterial community, as shown in the Mantel test. The co-occurrence network analysis unveiled intricate interactions among algae to denitrifying bacteria. Denitrifying bacterial community co-occurrence network complexity (larger average degrees representing stronger network complexity) increased continuously from July to September and decreased in October before increasing in November. The co-occurrence network complexity of the algae and nirS-encoding denitrifying bacteria tended to increase from July to November. The co-occurrence network complexity of the algal and denitrifying bacterial communities was modified by ammonia nitrogen (NH₄⁺-N) and total phosphorus (TP), pH, and water temperature (WT), according to the ordinary least-squares (OLS) model. The modified stochasticity ratio (MST) results reveal that deterministic selection dominated the assembly of denitrifying bacterial communities. The influence of environmental variables to denitrifying bacterial communities, as well as characteristics of algal-bacterial co-occurrence networks and the assembly process of denitrifying bacterial communities, were discovered in epiphytic biofilms in this study. The findings could aid in the appropriate understanding and use of epiphytic biofilms denitrification function, as well as the enhancement of water quality.

Key words: denitrifying bacteria epiphytic biofilms co-occurrence networks submerged macrophytes community assembly

Received: 2022-04-13 Revised:

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Articles by Guoqing LI

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Articles by Xin DU

Articles by Tao LIN

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