Environment drives the co-occurrence of bacteria and microeukaryotes in a typical subtropical bay -- Journal of Oceanology and Limnology,2023,41(6):2292-2308

	Cite this paper:
	Yifan MA, Lingfeng HUANG, Wenjing ZHANG. Environment drives the co-occurrence of bacteria and microeukaryotes in a typical subtropical bay[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2292-2308

Environment drives the co-occurrence of bacteria and microeukaryotes in a typical subtropical bay

Yifan MA^1,2,3, Lingfeng HUANG⁴, Wenjing ZHANG^1,2,3

1 State Key Laboratory of Marine Environmental Science, Marine Biodiversity and Global Change Research Center, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China;
2 Xiamen Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen University, Xiamen 361102, China;
3 Fujian Key Laboratory of Coastal Pollution Prevention and Control, Xiamen University, Xiamen 361102, China;
4 Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China

Abstract:

The co-occurrence of bacteria and microeukaryote species is a ubiquitous ecological phenomenon, but there is limited cross-domain research in aquatic environments. We conducted a network statistical analysis and visualization of microbial cross-domain co-occurrence patterns based on DNA sampling of a typical subtropical bay during four seasons, using high-throughput sequencing of both 18S rRNA and 16S rRNA genes. First, we found obvious relationships between network stability and network complexity indices. For example, increased cooperation and modularity were found to weaken the stability of cross-domain networks. Secondly, we found that bacterial operational taxonomic units (OTUs) were the most important contributors to network complexity and stability as they occupied more nodes, constituted more keystone OTUs, built more connections, more importantly, ignoring bacteria led to greater variation in network robustness. Gammaproteobacteria, Alphaproteobacteria, Bacteroidetes, and Actinobacteria were the most ecologically important groups. Finally, we found that the environmental drivers most associated with cross-domain networks varied across seasons (in detail, the network in January was primarily constrained by temperature and salinity, the network in April was primarily constrained by depth and temperature, the network in July was mainly affected by depth, temperature, and salinity, depth was the most important factor affecting the network in October) and that environmental influence was stronger on bacteria than on microeukaryotes.

Key words: co-occurrence network cross-domain network stability network complexity subtropical bay

Received: 2022-04-25 Revised:

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