<i>Makinoella parva</i>, a new species of the genus <i>Makinoella</i> (Oocystaceae, Trebouxiophyceae, Chlorophyta) -- Journal of Oceanology and Limnology,2023,41(6):2391-2402

	Cite this paper:
	Xudong LIU, Chen SU, Jia FENG, Junping LÜ, Qi LIU, Fangru NAN, Guoxiang LIU, Shulian XIE. Makinoella parva, a new species of the genus Makinoella (Oocystaceae, Trebouxiophyceae, Chlorophyta)[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2391-2402

Makinoella parva, a new species of the genus Makinoella (Oocystaceae, Trebouxiophyceae, Chlorophyta)

Xudong LIU¹, Chen SU¹, Jia FENG¹, Junping LÜ¹, Qi LIU¹, Fangru NAN¹, Guoxiang LIU², Shulian XIE¹

1 School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan 030006, China;
2 Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Abstract:

Makinoella Okada is a genus of coccoid green algae belonging to the family Oocystaceae with typical morphological characteristics. Reports to date included only one type species. Since the genus is rarely reported, there is a lack of taxonomic research. In this study, 17 strains of this genus were collected from several different places in China, and their taxonomic studies were conducted based on morphological and molecular phylogenetic analysis. The phylogenetic results based on the analysis of ITS, chloroplast genes rbcL and tufA showed that the genus was divided into two branches. One of the branches comprised newly collected algae. Compensatory base changes (CBCs) and hemi-compensatory base changes (hemi-CBCs) within the secondary structure of the ITS-2 confirmed the branches as two species. Compared with the type species of Makinoella tosaensis, the cell size of this new branch was only about 50%, and included 2–4 colonial cells. Therefore, based on the smaller cell size, simpler colony composition, independent phylogenetic position and CBCs and hemi-CBCs, we suggest that the new clade should be designated as a new taxon of Makinoella, namely Makinoella parva. Among the four molecular markers using in this study, the rbcL and newly introduced ITS are recommended for species separation, which can help further studies to revise the species and generic concepts of the family.

Key words: coccoid green algae|Makinoella|morphology observation|Oocystaceae|phylogenetic analysis

Received: 2022-07-23 Revised:

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References:
Bock C, Krienitz L, Pröschold T.2011.Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species.Fottea, 11(2):293-312, https://doi.org/10.5507/fot.2011.028.
Bock C, Luo W, Kusber W H et al.2013.Classification of crucigenoid algae:phylogenetic position of the reinstated genus Lemmermannia, Tetrastrum spp.Crucigenia tetrapedia, and C.lauterbornii (Trebouxiophyceae, Chlorophyta).Journal of Phycology, 49(2):329-339, https://doi.org/10.1111/jpy.12039.
Bourrelly P.1966.Les Algues Deau Douce, Initiation à la Systématique.Tome I:Les Algues Vertes.Ed.N.Boubée, Paris.512p.
Famà P, Wysor B, Kooistra W H C F et al.2002.Molecular phylogeny of the genus Caulerpa (Caulerpales, Chlorophyta) inferred from chloroplast tufA gene.Journal of Phycology, 38(5):1040-1050, https://doi.org/10.1046/j.1529-8817.2002.t01-1-01237.x.
Fukushima H.1956.A list of Japanese freshwater algae including the marine species of blue-green algae and fossil diatoms 2.Journal of the Yokohama Municipal University Series C, 46:1-12.
Hegewald E, Schnepf E, Jeon S L.1999.Report on Makinoella tosaensis Okada (Chlorophyta, Oocystaceae), a species new to Korea.Algae, 14(2):87-90.
Hepperle D, Hegewald E, Krienitz L.2000.Phylogenetic position of the Oocystaceae (Chlorophyta).Journal of Phycology, 36(3):590-595, https://doi.org/10.1046/j.1529-8817.2000.99184.x.
Hindák F, Hindáková A.2010.First report of Makinoella tosaensis OKADA (Chlorophyta, Chlorococcales, Oocystaceae) outside East Asia.Fottea, 10(1):141-144, https://doi.org/10.5507/fot.2010.008.
Iyengar M O P.1932.Two little-known genera of green algae (Tetrasporidium and Ecballocystis).Annals of Botany, 46(182):191-227.
Kalyaanamoorthy S, Minh B Q, Wong T K F et al.2017.ModelFinder:fast model selection for accurate phylogenetic estimates.Nature Methods, 14(6):587-589, https://doi.org/10.1038/nmeth.4285.
Komárek J, Fott B.1983.Chlorophyceae (Grünalgen) ordnung:chlorococcales.In:Huber-Pestalozzi G ed.Das Phytoplankton des Süsswassers:Systematik und Biologie.E.Schweizerbart'sche Verlagsbuchhandlung, Stuttgart.p.1-1044.
Krienitz L, Bock C, Kotut K et al.2012.Genotypic diversity of Dictyosphaerium-morphospecies (Chlorellaceae, Trebouxiophyceae) in African inland waters, including the description of four new genera.Fottea, 12(2):231-253, https://doi.org/10.5507/fot.2012.017.
Krienitz L, Bock C, Luo W et al.2010.Polyphyletic origin of the Dictyosphaerium morphotype within Chlorellaceae (Trebouxiophyceae).Journal of Phycology, 46(3):559-563, https://doi.org/10.1111/j.1529-8817.2010.00813.x.
Krienitz L, Hegewald E H, Hepperle D et al.2004.Phylogenetic relationship of Chlorella and Parachlorella gen.nov.(Chlorophyta, Trebouxiophyceae).Phycologia, 43(5):529-542, https://doi.org/10.2216/i0031-8884-43-5-529.1.
Larkin M A, Blackshields G, Brown N P et al.2007.Clustal W and Clustal X version 2.0.Bioinformatics, 23(21):2947-2948, https://doi.org/10.1093/bioinformatics/btm404.
Liu X D, Wang Q H, Zhu H et al.2020.Reticulocystis yunnanense gen.et sp.nov., a new member of freshwater Oocystaceae algae (Trebouxiophyceae, Chlorophyta).European Journal of Phycology, 55(4):507-516, https://doi.org/10.1080/09670262.2020.1751303.
Liu X D, Zhang R, Feng J et al.2021.Neglectella glomerata sp.nov., a new species and implications for the systematics of the genus Neglectella (Oocystaceae, Trebouxiophyceae, Chlorophyta).Journal of Oceanology and Limnology, 39(6):2370-2379, https://doi.org/10.1007/s00343-020-0356-3.
Liu X D, Zhu H, Liu B W et al.2017.Classification of planctonema-like algae, including a new genus Planctonemopsis gen.nov., a new species Planctonema gelatinosum sp.nov.and a reinstated genus Psephonema (Trebouxiophyceae, Chlorophyta).Journal of Phycology, 53(4):869-879, https://doi.org/10.1111/jpy.12551.
Liu X D, Zhu H, Song H Y et al.2018a.Euchlorocystis gen.nov.and Densicystis gen.nov., two new genera of Oocystaceae algae from high-altitude semi-saline habitat (Trebouxiophyceae, Chlorophyta).Journal of Eukaryotic Microbiology, 65(2):200-210, https://doi.org/10.1111/jeu.12455.
Liu X D, Zhu H, Song H Y et al.2018b.Quadricoccopsis gen.nov., a new genus of Quadricoccus-like algae in Oocystaceae from China (Trebouxiophyceae, Chlorophyta).Fottea, 18(2):189-199, https://doi.org/10.5507/fot.2018.005.
Luo W, Pflugmacher S, Pröschold T et al.2006.Genotype versus phenotype variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae).Protist, 157(3):315-333, https://doi.org/10.1016/j.protis.2006.05.006.
Luo W, Pröschold T, Bock C et al.2010.Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae).Plant Biology, 12(3):545-553, https://doi.org/10.1111/j.1438-8677.2009.00221.x.
Malavasi V, Škaloud P, Rindi F et al.2016.DNA-based taxonomy in ecologically versatile microalgae:a re-evaluation of the species concept within the coccoid green algal genus Coccomyxa (Trebouxiophyceae, Chlorophyta).PLoS One, 11(3):e0151137, https://doi.org/10.1371/journal.pone.0151137.
Mathews D H, Disney M D, Childs J L et al.2004.Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure.Proceedings of the National Academy of Sciences of the United States of America, 101(19):7287-7292, https://doi.org/10.1073/pnas.0401799101.
Montezinos D, Brown R M Jr.1978.Cell wall biogenesis in Oocystis:experimental alteration of microfibril assembly and orientation.Cytobios, 23(90):119-139.
Nguyen L T, Schmidt H A, Von Haeseler A et al.2015.IQ-TREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.Molecular Biology and Evolution, 32(1):268-274, https://doi.org/10.1093/molbev/msu300.
Okada Y.1949.Makinoella tosaensis, a new genus and species of Oocystaceae.The Journal of Japanese Botany, 24(1-12):166-168.(in Japanese with English abstract)
Řeháková H.1969.Die Variabilität der Arten der Gattung Oocystis A.Braun.Studies in Phycology.p.145-196.
Robinson D G, Preston R D.1972.Plasmalemma structure in relation to microfibril biosynthesis in Oocystis.Planta, 104(3):234-246.
Robinson D G, White R K.1972.The fine structure of Oocystis apiculata W.West with particular reference to the wall.British Phycological Journal, 7(1):109-118, https://doi.org/10.1080/00071617200650131.
Ronquist F, Teslenko M, van der Mark P et al.2012.MrBayes 3.2:efficient Bayesian phylogenetic inference and model choice across a large model space.Systematic Biology, 61(3):539-542, https://doi.org/10.1093/sysbio/sys029.
Sanders W B, Pérez-Ortega S, Nelsen M P et al.2016.Heveochlorella (Trebouxiophyceae):a little-known genus of unicellular green algae outside the trebouxiales emerges unexpectedly as a major clade of lichen photobionts in foliicolous communities.Journal of Phycology, 52(5):840-853, https://doi.org/10.1111/jpy.12446.
Schnepf E, Hegewald E.2000.The ultrastructure of Makinoella tosaensis OKADA (Chlorophyta, Oocystaceae).Algological Studies, 97:79-91, https://doi.org/10.1127/algol_stud/97/2000/79.
Schultz J, Müller T, Achtziger M et al.2006.The internal transcribed spacer 2 database-a web server for (not only) low level phylogenetic analyses.Nucleic Acids Research, 34(Suppl_2):W704-W707, https://doi.org/10.1093/nar/gkl129.
Sciuto K, Lewis L A, Verleyen E et al.2015.Chodatodesmus australis sp.nov.(Scenedesmaceae, Chlorophyta) from Antarctica, with the emended description of the genus Chodatodesmus, and circumscription of Flechtneria rotunda gen.et sp.nov.Journal of Phycology, 51(6):1172-1188, https://doi.org/10.1111/jpy.12355.
Seibel P N, Müller T, Dandekar T et al.2006.4SALE-a tool for synchronous RNA sequence and secondary structure alignment and editing.BMC Bioinformatics, 7:498, https://doi.org/10.1186/1471-2105-7-498.
Seibel P N, Müller T, Dandekar T et al.2008.Synchronous visual analysis and editing of RNA sequence and secondary structure alignments using 4SALE.BMC Research Notes, 1:91, https://doi.org/10.1186/1756-0500-1-91.
Song H Y, Liu X D, Hu Y X et al.2018.Coronacoccus hengyangensis gen.et sp.nov., a new member of Chlorellaceae (Trebouxiophyceae, Chlorophyta) with radiococcacean morphology.Phycologia, 57(4):363-373, https://doi.org/10.2216/17-65.1.
Song H Y, Zhang Q, Liu G X et al.2015.Polulichloris henanensis gen.et sp.nov.(Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga.Phytotaxa, 218(2):137-146, https://doi.org/10.11646/phytotaxa.218.2.3.
Štenclová L, Fučíková K, Kaštovský J et al.2017.Molecular and morphological delimitation and generic classification of the family Oocystaceae (Trebouxiophyceae, Chlorophyta).Journal of Phycology, 53(6):1263-1282, https://doi.org/10.1111/jpy.12581.
Stoyneva M P, Cocquyt C, Gärtner G et al.2007.Oocystis lacustris CHOD.(Chlorophyta, Trebouxiophyceae) in Lake Tanganyika (Africa).Linzer Biologische Beitrage, 39(1):571-632.
Tamura K, Peterson D, Peterson N et al.2011.MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Molecular Biology and Evolution, 28(10):2731-2739, https://doi.org/10.1093/molbev/msr121.
Vieira H H, Bagatini I L, Guinart C M et al.2016.tufA gene as molecular marker for freshwater Chlorophyceae.Algae, 31(2):155-165, https://doi.org/10.4490/algae.2016.31.4.14.
Wang Q H, Liu X D, Li S Y et al.2020.Cryptic species inside the genus Hariotina (Scenedesmaceae, Sphaeropleales), with descriptions of four new species in this genus.European Journal of Phycology, 55(4):373-383, https://doi.org/10.1080/09670262.2020.1737968.
Wang Q H, Song H Y, Liu X D et al.2019.Morphology and molecular phylogeny of coccoid green algae Coelastrella sensu lato (Scenedesmaceae, Sphaeropeales), including the description of three new species and two new varieties.Journal of Phycology, 55(6):1290-1305, https://doi.org/10.1111/jpy.12915.
West W, West G S.1898.Notes on freshwater algae.Journal of Botany, British and Foreign, 36:330-338.
Xia S, Zhu H, Cheng Y Y et al.2013.Phylogenetic position of Ecballocystis and Ecballocystopsis (Chlorophyta).Fottea, 13(1):65-75, https://doi.org/10.5507/fot.2013.006.
Yamagishi T, Akiyama M.1984.Photomicrographs of the Freshwater Algae.Uchida Rokakuho Publishing Co., Tokyo.58p.
Zhang Q, Song H Y, Zheng L L et al.2016.Makinoella tosaensis Okada, a new recorded genus and species of freshwater Trebouxiophycean algae from China.Journal of Tropical and Subtropical Botany, 24(4):406-412, https://doi.org/10.11926/j.issn.1005-3395.2016.04.007.(in Chinese with English abstract)