Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary -- Journal of Oceanology and Limnology,2023,41(6):2418-2428

	Cite this paper:
	Yudong JIA, Yunhong GAO, Jinxing LIN. Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2418-2428

Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary

Yudong JIA¹, Yunhong GAO¹, Jinxing LIN²

1 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Shanghai Laboratory Animal Research Center, Shanghai 201203, China

Abstract:

Reproduction is subtlety regulated by the hypothalamic-pituitary-gonad (HPG) axis in vertebrates. Pituitary gland is the center of the HPG axis, while pituitary gonadotropins follicle stimulating hormone (FSH) and luteinizing hormone (LH) were identified the key elements of the HPG axis in teleost and mammal. Morphology, cell lines, and gonadotropins cell localization of female turbot and mouse pituitary were determined at mature stage to illustrate the anatomical difference and cell characteristics in this study. Results show that turbot pituitary is chicken heart-shaped, dorsoventral, located on the ventral surface of the diencephalon. The mouse pituitary is oval, located in the pituitary fossa of the sella turcica at the skull base. Two well-distinguished areas adenohypophysis (AH) and neurohypophysis (NH) in pituitary were identified in turbot and mouse. Turbot AH comprised the rostral pars distalis (RPD), proximal pars distalis (PPD), and pars intermedia (PI). NH was not pronounced and with finger-like protrusions into PPD. However, mouse AH only comprised the pars distalis (PD) and PI. NH distribution was semicircular. Three main types of cells (acidophilic, basophilic, and chromophobic cells) were distributed in the mouse PD region, whereas appeared in the turbot PPD, RPD, and PI. Moreover, the percentage of mouse chromophobic and basophilic cells was higher and lower than that of turbot, respectively. The diameter of the aforementioned three cells in the mouse was significantly higher than turbot. fshβ- and lhβ-immunoreactive signals were identified in turbot-distinct pituitary cells that primarily occupied the peripheral and central regions of AH. However, mouse fsh- and lh-immunoreactive cells were expressed in the same cells and present in the PD. These results demonstrate the significantly difference of pituitary morphology, cell lines and gonadotropins (fshβ and lhβ) location in female turbot and mouse. These differences help for fully understand the evolution and endocrinological functions of pituitary.

Key words: turbot|mouse|pituitary gland|cell line|gonadotropins

Received: 2022-06-20 Revised:

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References:
Agulleiro B, García H M P, García A A.2006.Teleost adenohypophysis:morphofunctional and developmental aspects.In:Reinecke M, Zaccone G, Kapoor B G eds.Fish Endocrinology.Science Publishers, Enfield.p.290-324.
Amar A P, Weiss M H.2003.Pituitary anatomy and physiology.Neurosurgery Clinics of North America, 14(1):11-23, https://doi.org/10.1016/S1042-3680(02)00017-7.
Bancroft J D, Stevens A.1982.Theory and Practice of Histological Techniques.2nd edn.Churchill Livingstone, New York.
Borella M I, Venturieri R, Mancera J M.2009.Immunocytochemical identification of adenohypophyseal cells in the pirarucu (Arapaima gigas), an Amazonian basal teleost.Fish Physiology and Biochemistry, 35(1):3-16, https://doi.org/10.1007/s10695-008-9254-x.
Cerdá-Reverter J M, Canosa L F.2009.Neuroendocrine systems of the fish brain.Fish Physiology, 28:3-74, https://doi.org/10.1016/S1546-5098(09)28001-0.
Chen W T, Ge W.2012.Ontogenic expression profiles of gonadotropins (fshb and lhb) and growth hormone (gh) during sexual differentiation and puberty onset in female zebrafish.Biology of Reproduction, 86(3):73, https://doi.org/10.1095/biolreprod.111.094730.
Davis S W, Keisler J L, Pérez-Millán M I et al.2016.All hormone-producing cell types of the pituitary intermediate and anterior lobes derive from prop1-expressing progenitors.Endocrinology, 157(4):1385-1396, https://doi.org/10.1210/en.2015-1862.
D'Este L, Casini A, Cetin Y et al.2005.Guanylin-immunoreactive cells in the female and male rat adenohypophysis and their changes under various physiological and experimental conditions.Histochemistry and Cell Biology, 123(3):303-313, https://doi.org/10.1007/s00418-004-0738-1.
El-Sakhawy M A, El-Shamma M A, Rabou M I A et al.2011.Seasonal histology and histochemistry of the adenohypophysis of nile tilapia (Oreochromis niloticus).Journal of Veterinary Anatomy, 4(2):39-60, https://doi.org/10.21608/jva.2011.45307.
Fontaine R, Ciani E, Haug T M et al.2020.Gonadotrope plasticity at cellular, population and structural levels:a comparison between fishes and mammals.General and Comparative Endocrinology, 287:113344, https://doi.org/10.1016/j.ygcen.2019.113344
Gao Y H, Jing Q Q, Huang B et al.2019.Molecular cloning, characterization, and mRNA expression of gonadotropins during larval development in turbot (Scophthalmus maximus).Fish Physiology and Biochemistry, 45(5):1697-1707, https://doi.org/10.1007/s10695-019-00656-z.
Golan M, Martin A O, Mollard P et al.2016.Anatomical and functional gonadotrope networks in the teleost pituitary.Scientific Reports, 6(1):23777, https://doi.org/10.1038/srep23777.
Grandi G, Marchetti M G, Lanzoni M et al.2014.Immunocytochemical and ultrastructural identification of adenohypophyseal cells in Ctenopharyngodon idella (Cypriniformes:Cyprinidae) during gonadal differentiation.Fish Physiology and Biochemistry, 40(4):1115-1139, https://doi.org/10.1007/s10695-014-9910-2.
Guzmán J M, Luckenbach J A, Swanson P.2013.Molecular characterization and quantification of sablefish (Anoplopoma fimbria) gonadotropins and their receptors:reproductive dysfunction in female captive broodstock.General and Comparative Endocrinology, 193:37-47, https://doi.org/10.1016/j.ygcen.2013.07.007.
Herzog W, Zeng X C, Lele Z et al.2003.Adenohypophysis formation in the zebrafish and its dependence on sonic hedgehog.Developmental Biology, 254(1):36-49, https://doi.org/10.1016/S0012-1606(02)00124-0.
Holmes R L, Ball J N.1974.The Pituitary Gland:A Comparative Account.Cambridge University Press, London.
Hong G K, Payne S C, Jane J A Jr.2016.Anatomy, physiology, and laboratory evaluation of the pituitary gland.Otolaryngologic Clinics of North America, 49(1):21-32, https://doi.org/10.1016/j.otc.2015.09.002.
Honji R M, Nóbrega R H, Pandolfi M et al.2013.Immunohistochemical study of pituitary cells in wild and captive salminus hilarii (characiformes:characidae) females during the annual reproductive cycle.SpringerPlus, 2(1):460, https://doi.org/10.1186/2193-1801-2-460.
Hurvitz A, Degani G, Goldberg D et al.2005.Cloning of FSHβ, LHβ, and glycoprotein α subunits from the Russian Sturgeon (Acipenser gueldenstaedtii), β-subunit mRNA expression, gonad development, and steroid levels in immature fish.General and Comparative Endocrinology, 140(1):61-73, https://doi.org/10.1016/j.ygcen.2004.09.019.
Ifft J D.1953.The effect of superior cervical ganglionectomy on the cell population of the rat adenohypophysis and on the estrous cycle.The Anatomical Record, 117(3):395-404, https://doi.org/10.1002/ar.1091170305.
Japon M A, Rubinstein M, Low M J.1994.In situ hybridization analysis of anterior pituitary hormone gene expression during fetal mouse development.Journal of Histochemistry & Cytochemistry, 42(8):1117-1125, https://doi.org/10.1177/42.8.8027530.
Jia Y D, Lei J L.2019.Molecular function of gonadotrophins and their receptors in the ovarian development of turbot (Scophthalmus maximus).General and Comparative Endocrinology, 277:17-19, https://doi.org/10.1016/j.ygcen.2019.02.014.
Jia Y D, Meng Z, Niu H X et al.2014.Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus).Fish Physiology and Biochemistry, 40(6):1639-1650, https://doi.org/10.1007/s10695-014-9954-3.
Jia Y D, Sun A, Meng Z et al.2016.Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus).Fish Physiology and Biochemistry, 42(1):179-191, https://doi.org/10.1007/s10695-015-0128-8.
Kasper R S, Shved N, Takahashi A et al.2006.A systematic immunohistochemical survey of the distribution patterns of GH, prolactin, somatolactin, β-TSH, β-FSH, β-LH, ACTH, and α-MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia.Cell and Tissue Research, 325(2):303-313, https://doi.org/10.1007/s00441-005-0119-7.
Levavi-Sivan B, Bogerd J, Mañanós E L et al.2010.Perspectives on fish gonadotropins and their receptors.General and Comparative Endocrinology, 165(3):412-437, https://doi.org/10.1016/j.ygcen.2009.07.019.
Mattheij J A M, Stroband H W J, Kingma F J.1971.The cell types in the adenohypophysis of the cichlid fish Cichlasoma biocellatum regan, with special attention to its osmoregulatory role.Zeitschrift fur Zellforschung und mikroskopische Anatomie, 118(1):113-126, https://doi.org/10.1007/BF00331770.
Mazón M J, Molés G, Rocha A et al.2015.Gonadotropins in European sea bass:endocrine roles and biotechnological applications.General and Comparative Endocrinology, 221:31-41, https://doi.org/10.1016/j.ygcen.2015.05.002.
Mollard P, Hodson D J, Lafont C et al.2012.A tridimensional view of pituitary development and function.Trends in Endocrinology & Metabolism, 23(6):261-269, https://doi.org/10.1016/j.tem.2012.02.004.
Musumeci G, Castorina S, Castrogiovanni P et al.2015.A journey through the pituitary gland:development, structure and function, with emphasis on embryo-foetal and later development.Acta Histochemica, 117(4-5):355-366, https://doi.org/10.1016/j.acthis.2015.02.008.
Norris D O.2007.Vertebrate Endocrinology, 4th edn.Academic Press.p.549.
Ooi G T, Tawadros N, Escalona R M.2004.Pituitary cell lines and their endocrine applications.Molecular and Cellular Endocrinology, 228(1-2):1-21, https://doi.org/10.1016/j.mce.2004.07.018.
Pogoda H M, Hammerschmidt M.2009.How to make a teleost adenohypophysis:molecular pathways of pituitary development in zebrafish.Molecular and Cellular Endocrinology, 312(1-2):2-13, https://doi.org/10.1016/j.mce.2009.03.012.
Shimizu A, Tanaka H, Kagawa H.2003.Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog gth subunits:examining seasonal variations of gonadotrophs (fsh cells and lh cells) in the mummichog and applications to other acanthopterygian fishes.General and Comparative Endocrinology, 132(1):35-45, https://doi.org/10.1016/S0016-6480(03)00037-6.
Weltzien F A, Andersson E, Andersen Ø et al.2004.The brain-pituitary-gonad axis in male teleosts, with special emphasis on flatfish (Pleuronectiformes).Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 137(3):447-477, https://doi.org/10.1016/j.cbpb.2003.11.007.
Weltzien F A, Hildahl J, Hodne K et al.2014.Embryonic development of gonadotrope cells and gonadotropic hormones-lessons from model fish.Molecular and Cellular Endocrinology, 385(1-2):18-27, https://doi.org/10.1016/j.mce.2013.10.016.
Weltzien F A, Norberg B, Helvik J V et al.2003.Identification and localization of eight distinct hormone-producing cell types in the pituitary of male Atlantic halibut (Hippoglossus hippoglossus L.).Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 134(2):315-327, https://doi.org/10.1016/S1095-6433(02)00266-0.
Yoshie S, Honma Y.1978.Experimental demonstration of the cell types in the adenohypophysis of the gobiid fish, Rhinogobius brunneus.Archivum Histologicum Japonicum, 41(2):129-140, https://doi.org/10.1679/aohc1950.41.129.
Zhang Z W, Lau S W, Zhang L L et al.2015.Disruption of zebrafish follicle-stimulating hormone receptor (fshr) but not luteinizing hormone receptor (lhcgr) gene by TALEN leads to failed follicle activation in females followed by sexual reversal to males.Endocrinology, 156(10):3747-3762, https://doi.org/10.1210/en.2015-1039.
Zhu X Y, Gleiberman A S, Rosenfeld M G.2007.Molecular physiology of pituitary development:signaling and transcriptional networks.Physiological Reviews, 87(3):933-963, https://doi.org/10.1152/physrev.00006.2006.