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Yudong JIA, Yunhong GAO, Jinxing LIN. Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2418-2428

Morphological comparison and gonadotropins cell localization of mature female turbot and mouse pituitary

Yudong JIA1, Yunhong GAO1, Jinxing LIN2
1 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Shanghai Laboratory Animal Research Center, Shanghai 201203, China
Reproduction is subtlety regulated by the hypothalamic-pituitary-gonad (HPG) axis in vertebrates. Pituitary gland is the center of the HPG axis, while pituitary gonadotropins follicle stimulating hormone (FSH) and luteinizing hormone (LH) were identified the key elements of the HPG axis in teleost and mammal. Morphology, cell lines, and gonadotropins cell localization of female turbot and mouse pituitary were determined at mature stage to illustrate the anatomical difference and cell characteristics in this study. Results show that turbot pituitary is chicken heart-shaped, dorsoventral, located on the ventral surface of the diencephalon. The mouse pituitary is oval, located in the pituitary fossa of the sella turcica at the skull base. Two well-distinguished areas adenohypophysis (AH) and neurohypophysis (NH) in pituitary were identified in turbot and mouse. Turbot AH comprised the rostral pars distalis (RPD), proximal pars distalis (PPD), and pars intermedia (PI). NH was not pronounced and with finger-like protrusions into PPD. However, mouse AH only comprised the pars distalis (PD) and PI. NH distribution was semicircular. Three main types of cells (acidophilic, basophilic, and chromophobic cells) were distributed in the mouse PD region, whereas appeared in the turbot PPD, RPD, and PI. Moreover, the percentage of mouse chromophobic and basophilic cells was higher and lower than that of turbot, respectively. The diameter of the aforementioned three cells in the mouse was significantly higher than turbot. fshβ- and lhβ-immunoreactive signals were identified in turbot-distinct pituitary cells that primarily occupied the peripheral and central regions of AH. However, mouse fsh- and lh-immunoreactive cells were expressed in the same cells and present in the PD. These results demonstrate the significantly difference of pituitary morphology, cell lines and gonadotropins (fshβ and lhβ) location in female turbot and mouse. These differences help for fully understand the evolution and endocrinological functions of pituitary.
Key words:    turbot|mouse|pituitary gland|cell line|gonadotropins   
Received: 2022-06-20   Revised:
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