Environmental hypoxia induces apoptosis in large yellow croaker <i>Larimichthys crocea</i> via both intrinsic and extrinsic pathways -- Journal of Oceanology and Limnology,2023,41(6):2429-2443

	Cite this paper:
	Shengyu LUO, Cheng LIU, Xinming GAO, Jingqian WANG, Yibo ZHANG, Jie DING, Congcong HOU, Junquan ZHU, Bao LOU, Weiliang SHEN, Xiongfei WU, Chundan ZHANG. Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways[J]. Journal of Oceanology and Limnology, 2023, 41(6): 2429-2443

Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways

Shengyu LUO¹, Cheng LIU¹, Xinming GAO¹, Jingqian WANG¹, Yibo ZHANG¹, Jie DING¹, Congcong HOU¹, Junquan ZHU¹, Bao LOU², Weiliang SHEN³, Xiongfei WU³, Chundan ZHANG¹

1 Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3 State Key Laboratory of Large Yellow Croaker Breeding, Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China

Abstract:

Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea, an economically important mariculture fish in China. Apoptosis is a consequence of hypoxia on fish. However, the effects of hypoxia stress on apoptosis in L. crocea remain largely unknown. We investigated the effect of environmental hypoxia on apoptosis in L. crocea. Results show that hypoxia induced apoptosis in L. crocea both in vivo and in vitro. The mitochondrial membrane potential was significantly reduced in large yellow croaker fry (LYCF) cells. The expression levels of B-cell lymphoma/leukemia-2 (Bcl-2) mRNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress, respectively, whereas the expression level of Bcl-2 associated X (Bax) mRNA, Casp3 mRNA, and activity of caspase-3/7/9 were significantly increased, indicating that hypoxia induced caspase-dependent intrinsic apoptosis in L. crocea. The expression level of the apoptosis-inducing factor (AIF) protein was significantly increased in the liver and LYCF cells. The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of L. crocea, demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis. In addition, the activity of caspase-8 was significantly increased, indicating that hypoxia stress induced extrinsic apoptosis in L. crocea. Therefore, hypoxia induced apoptosis in L. crocea through both the intrinsic and extrinsic pathways. The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

Key words: hypoxia|large yellow croaker|mitochondrial apoptosis pathway|death receptor apoptosis pathway

Received: 2022-07-04 Revised:

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Articles by Shengyu LUO

Articles by Cheng LIU

Articles by Xinming GAO

Articles by Jingqian WANG

Articles by Yibo ZHANG

Articles by Jie DING

Articles by Congcong HOU

Articles by Junquan ZHU

Articles by Bao LOU

Articles by Weiliang SHEN

Articles by Xiongfei WU

Articles by Chundan ZHANG

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