Complete nuclear ribosomal DNA sequence amplification and molecular analyses of <i>Bangia</i> (<i>Bangia</i>les, Rhodophyta) from China -- Journal of Oceanology and Limnology,2016,34(5):1044-1053

	Cite this paper:
	XU Jiajie, JIANG Bo, CHAI Sanming, HE Yuan, ZHU Jianyi, SHEN Zonggen, SHEN Songdong. Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China[J]. Journal of Oceanology and Limnology, 2016, 34(5): 1044-1053

Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China

XU Jiajie¹, JIANG Bo², CHAI Sanming³, HE Yuan¹, ZHU Jianyi², SHEN Zonggen², SHEN Songdong¹

1 Department of Cell Biology, College of Biology and Basic Medical Sciences, Soochow University, SIP, Suzhou 215123, China;
2 Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China;
3 Maternal and Child Health Hospital of Gansu Province, Lanzhou 730050, China

Abstract:

Filamentous Bangia, which are distributed extensively throughout the world, have simple and similar morphological characteristics. Scientists can classify these organisms using molecular markers in combination with morphology. We successfully sequenced the complete nuclear ribosomal DNA, approximately 13 kb in length, from a marine Bangia population. We further analyzed the small subunit ribosomal DNA gene (nrSSU) and the internal transcribed spacer (ITS) sequence regions along with nine other marine, and two freshwater Bangia samples from China. Pairwise distances of the nrSSU and 5.8S ribosomal DNA gene sequences show the marine samples grouping together with low divergences (0-0.003; 0-0.006, respectively) from each other, but high divergences (0.123-0.126; 0.198, respectively) from freshwater samples. An exception is the marine sample collected from Weihai, which shows high divergence from both other marine samples (0.063-0.065; 0.129, respectively) and the freshwater samples (0.097; 0.120, respectively). A maximum likelihood phylogenetic tree based on a combined SSU-ITS dataset with maximum likelihood method shows the samples divided into three clades, with the two marine sample clades containing Bangia spp. from North America, Europe, Asia, and Australia; and one freshwater clade, containing Bangia atropurpurea from North America and China.

Key words: Bangia|molecular analysis|small subunit ribosomal DNA gene (nrSSU)|internal transcribed spacer (ITS)|ribosomal DNA

Received: 2015-03-16 Revised: 2015-06-30

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