Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass -- Journal of Oceanology and Limnology,2015,33(2):328-338

	Cite this paper:
	LI Shengjie, HAN Linqiang, BAI Junjie, MA Dongmei, QUAN Yingchun, FAN Jiajia, JIANG Peng, YU Lingyun. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass[J]. Journal of Oceanology and Limnology, 2015, 33(2): 328-338

Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

LI Shengjie, HAN Linqiang, BAI Junjie, MA Dongmei, QUAN Yingchun, FAN Jiajia, JIANG Peng, YU Lingyun

Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, China Ministry of Agriculture, Guangzhou 510380, China

Abstract:

Pituitary adenylate cyclase activating polypeptide (PACAP) has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide (PRP) from the brain of largemouth bass (Micropterus salmoides) and used real-time quantitative PCR to detect PRP-PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing: a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-long and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch (dph). The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP-PACAP acts as an important factor in appetite regulation in largemouth bass.

Key words: pituitary adenylate cyclase activating polypeptide (PACAP)|PACAP-related peptide|largemouth bass|gene expression|fasting

Received: 2014-05-05 Revised: 2014-06-28

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Articles by LI Shengjie

Articles by HAN Linqiang

Articles by BAI Junjie

Articles by MA Dongmei

Articles by QUAN Yingchun

Articles by FAN Jiajia

Articles by JIANG Peng

Articles by YU Lingyun

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