Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea -- Journal of Oceanology and Limnology,2018,36(3):783-794

	Cite this paper:
	TENG Zhaojie, ZHANG Wenyan, CHEN Yiran, PAN Hongmiao, XIAO Tian, WU Long-Fei. Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea[J]. Journal of Oceanology and Limnology, 2018, 36(3): 783-794

Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea

TENG Zhaojie^1,2,3, ZHANG Wenyan^1,3, CHEN Yiran^1,3, PAN Hongmiao^1,3, XIAO Tian^1,3, WU Long-Fei^4,5

1 CAS Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China;
4 Laboratoire de Chimie Bactérienne, UMR 7283, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université, CNRS, Marseille, France;
5 Laboratoire International Associé de la Bio-Minéralisation et Nano-Structures, CNRS, Marseille, France

Abstract:

Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, coccoid, rod, vibrio, and multicellular morphotypes. The coccid shape is generally the most abundant morphotype among magnetotactic bacteria. Here we describe a species of giant rod-shaped magnetotactic bacteria (designated QR-1) collected from sediment in the low tide zone of Huiquan Bay (Yellow Sea, China). This morphotype accounted for 90% of the magnetotactic bacteria collected, and the only taxonomic group which was detected in the sampling site. Microscopy analysis revealed that QR-1 cells averaged (6.71±1.03)×(1.54±0.20) μm in size, and contained in each cell 42-146 magnetosomes that are arranged in a bundle formed one to four chains along the long axis of the cell. The QR-1 cells displayed axial magnetotaxis with an average velocity of 70±28 μm/s. Transmission electron microscopy based analysis showed that QR-1 cells had two tufts of flagella at each end. Phylogenetic analysis of the 16S rRNA genes revealed that QR-1 together with three other rod-shaped uncultivated magnetotactic bacteria are clustered into a deep branch of Alphaproteobacteria.

Key words: Alphaproteobacteria|flagella|motility|rod-shaped magnetotactic bacteria

Received: 2017-03-07 Revised:

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