The first complete organellar genomes of an Antarctic red alga, <i>Pyropia endiviifolia</i>: insights into its genome architecture and phylogenetic position within genus <i>Pyropia</i> (Bangiales, Rhodophyta) -- Journal of Oceanology and Limnology,2018,36(4):1315-1328

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	XU Kuipeng, TANG Xianghai, BI Guiqi, CAO Min, WANG Lu, MAO Yunxiang. The first complete organellar genomes of an Antarctic red alga, Pyropia endiviifolia: insights into its genome architecture and phylogenetic position within genus Pyropia (Bangiales, Rhodophyta)[J]. Journal of Oceanology and Limnology, 2018, 36(4): 1315-1328

The first complete organellar genomes of an Antarctic red alga, Pyropia endiviifolia: insights into its genome architecture and phylogenetic position within genus Pyropia (Bangiales, Rhodophyta)

XU Kuipeng¹, TANG Xianghai¹, BI Guiqi¹, CAO Min¹, WANG Lu¹, MAO Yunxiang^1,2

1 Key Laboratory of Marine Genetics and Breeding(Ocean University of China), Ministry of Education, Qingdao 266003, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China

Abstract:

Pyropia species grow in the intertidal zone and are cold-water adapted. To date, most of the information about the whole plastid and mitochondrial genomes (ptDNA and mtDNA) of this genus is limited to Northern Hemisphere species. Here, we report the sequencing of the ptDNA and mtDNA of the Antarctic red alga Pyropia endiviifolia using the Illumina platform. The plastid genome (195 784 bp, 33.28% GC content) contains 210 protein-coding genes, 37 tRNA genes and 6 rRNA genes. The mitochondrial genome (34 603 bp, 30.5% GC content) contains 26 protein-coding genes, 25 tRNA genes and 2 rRNA genes. Our results suggest that the organellar genomes of Py. endiviifolia have a compact organization. Although the collinearity of these genomes is conserved compared with other Pyropia species, the genome sizes show significant differences, mainly because of the different copy numbers of rDNA operons in the ptDNA and group Ⅱ introns in the mtDNA. The other Pyropia species have 2-3 distinct intronic ORFs in their cox1 genes, but Py. endiviifolia has no introns in its cox1 gene. This has led to a smaller mtDNA than in other Pyropia species. The phylogenetic relationships within Pyropia were examined using concatenated gene sets from most of the available organellar genomes with both the maximum likelihood and Bayesian methods. The analysis revealed a sister taxa affiliation between the Antarctic species Py. endiviifolia and the North American species Py. kanakaensis.

Key words: Antarctic|Pyropia endiviifolia|plastid and mitochondrial genomes|genome structure|phylogenetic

Received: 2017-03-20 Revised:

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