Gene-associated microsatellite markers confirm panmixia and indicate a different pattern of spatially varying selection in the endangered Japanese eel <i>Anguilla japonica</i> -- Journal of Oceanology and Limnology,2020,38(5):1572-1583

	Cite this paper:
	YU Lei, LIU Yanfang, LIU Jinxian. Gene-associated microsatellite markers confirm panmixia and indicate a different pattern of spatially varying selection in the endangered Japanese eel Anguilla japonica[J]. Journal of Oceanology and Limnology, 2020, 38(5): 1572-1583

Gene-associated microsatellite markers confirm panmixia and indicate a different pattern of spatially varying selection in the endangered Japanese eel Anguilla japonica

YU Lei^1,2,3,4, LIU Yanfang^1,2,3,4, LIU Jinxian^1,3,4

1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China

Abstract:

The Japanese eel (Anguilla japonica) is a commercially important fish species in East Asia and its recruitment has been rapidly declining since 1990s. Clarifying the genetic population structure of A. japonica is the basis of multinational cooperation on its management and protection, due to its large distribution range. Gene-associated markers have been proved powerful in delineating fine-scale population genetic structure and spatially varying selection. In the present study, we developed 24 polymorphic gene-associated microsatellite markers including 18 loci associated with the genes under selection in the two North Atlantic eel species (Anguilla anguilla and Anguilla rostrata) and 6 loci based on transcript sequences. A total of 13 geographic populations were sampled across its distribution range, including 11 samples from China (9 from China's mainland and 2 from Taiwan region), and 2 samples from Japan. A total of 416 individuals (mostly glass eels) were collected and genotyped at the 24 microsatellites. All measures of differentiation were accordant with a panmictic scenario (F_ST=-0.001) in A. japonica. No footprints of spatially varying selection were found, indicating that the selection pattern in A. japonica might be different from that in the two North Atlantic eel species. We suggest that A. japonica should be managed as a single unit and management and conservation efforts must be coordinated at the international level, as overexploitation in any region will decrease its recruitment across the whole distributional range.

Key words: Japanese eel|Anguilla japonica|panmixia|gene-associated microsatellite|spatially varying selection

Received: 2020-02-10 Revised: 2020-03-26

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