Bacterial diversity in the intestine of young farmed puffer fish <i>Takifugu rubripes</i> -- Journal of Oceanology and Limnology,2015,33(4):913-918

	Cite this paper:
	LI Yanyu, ZHANG Tao, ZHANG Congyao, ZHU Ying, DING Jianfeng, MA Yuexin. Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes[J]. Journal of Oceanology and Limnology, 2015, 33(4): 913-918

Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes

LI Yanyu¹, ZHANG Tao², ZHANG Congyao¹, ZHU Ying¹, DING Jianfeng¹, MA Yuexin¹

1 Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;
2 Dalian Tianzheng Industry Co. Ltd., Dalian 116011, China

Abstract:

The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16S rDNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216E medium. All the isolates were classified into two phyla—Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota (68.3%). At the genus level, Vibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and Shewanella were isolated from the intestine, with representatives of the genera Vibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.

Key words: 16S rDNA|biodiversity|intestinal microflora|Takifugu rubripes

Received: 2014-09-03 Revised: 2015-01-20

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